SR STED Confocal
Location: Building E, room 006a
Contact person: Dr. Michael Assa
Booking
Description
Leica SPi 8 Super-Resolution gSTED Inverted Confocal Microscope
The Leica 3D gSTED system achieves resolution below the diffraction limit in the lateral, and axial directions.
There are 3 gSTED continuous wave lasers at 592 nm (CW), 660 nm (CW) and 775 nm (pulsing laser) which allow the use of fluorophores From 470 to 670 nm in super-resolution.
Manufacturer Features
- Manufacturer: Leica
- Model: Inverted Leica DMi8 with anti-vibration table
- Continuous wave laser: UV – Diode 50mW 405nm
- Pulsed laser: White Light Laser (WLL). Avg. power 1.5mW: 470 – 670nm
- Depletion lasers: two continuous (CW) wave lasers up to 1.5W at 592 nm, 660 nm and wave pulsing laser at 775 nm 80 MHz (Class 4 lasers)
- Spectral Detection Range of 400nm – 720nm
- Detectors: 3 HyD (hybrid detectors) Gated, 2 PMTs, and a TLD (transmitted light detector)
- Excitation Modulation (Filters): up to 8 channels (filters multi-photon ext. & ems.); Acousto Optical Beam Splitter (AOBS): provides selectivity between 0.6 & 2 nm wavelength (no filter set needed).
- Resonant Scanner 8kHz
- Accelerated Z-stacking with the SuperZ galvanometer substage
- 3-D STED (up to 70 nm in Z)
- HyVolution – SuperResolution for every experiment and every sample (down to 140nm in XY).
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Technical details and protocols
