STED Technical details and protocols

Location: Building E, room 006A (SR room)

Reservation: BookIt

Tutoring: First time users must order a tutoring session.

Objectives:

  • HC PL FLUOTAR 10x/0.30Plan Fluotar objective, for use with and without coverglass, no immersion.Free working distance: 11.0 mm.
  • HC PL APO 20x/0.75 CS2Plan apochromat objective, no immersion.Free working distance: 0.62 mm.
  • HC PL APO 63x/1.40 OIL CS2Plan apochromat oil immersion objective.Free working distance: 0.14 mm.
  • C PL APO 93X/1.30 GLYC motCORR  STED objective with glycerol immersionMotorized correction collarFree working distance: 0.3 mm.
  • HC PL APO 100x/1.40 Oil STED onlyPlan apochromat oil immersion objective especially designed for STED.Free working distance: 0.13 mm.

Site http://www.leica-microsystems.com

https://www.leica-microsystems.com/science-lab/hyvolution-super-resolut… -->Sample preparation User Manual Sample

preparation: Sample preparation guide

User Manual: SP8 STED

 

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