STED Technical details and protocols
Location: Building E, room 006A (SR room)
Reservation: BookIt
Tutoring: First time users must order a tutoring session.
Objectives:
- HC PL FLUOTAR 10x/0.30Plan Fluotar objective, for use with and without coverglass, no immersion.Free working distance: 11.0 mm.
- HC PL APO 20x/0.75 CS2Plan apochromat objective, no immersion.Free working distance: 0.62 mm.
- HC PL APO 63x/1.40 OIL CS2Plan apochromat oil immersion objective.Free working distance: 0.14 mm.
- C PL APO 93X/1.30 GLYC motCORR STED objective with glycerol immersionMotorized correction collarFree working distance: 0.3 mm.
- HC PL APO 100x/1.40 Oil STED onlyPlan apochromat oil immersion objective especially designed for STED.Free working distance: 0.13 mm.
Site http://www.leica-microsystems.com
https://www.leica-microsystems.com/science-lab/hyvolution-super-resolut… -->Sample preparation User Manual Sample
preparation: Sample preparation guide
User Manual: SP8 STED