SR STED Confocal

Location: Building E, room 006a

Contact person: Dr. Michael Assa


SR STED Confocal


Leica SPi 8 Super-Resolution gSTED Inverted Confocal Microscope

The Leica 3D gSTED system achieves resolution below the diffraction limit in the lateral, and axial directions. There are 3 gSTED continuous wave lasers at 592 nm (CW), 660 nm (CW) and 775 nm (pulsing laser) which allow the use of fluorophores  From 470 to 670 nm in super-resolution.

Manufacturer Features

  • Manufacturer: Leica
  • Model: Inverted Leica DMi8 with anti-vibration table
  • Continuous wave laser: UV – Diode 50mW 405nm
  • Pulsed laser: White Light Laser (WLL). Avg. power 1.5mW: 470 – 670nm
  • Depletion lasers: two continuous (CW) wave lasers up to 1.5W at 592 nm, 660 nm and wave pulsing laser at 775 nm 80 MHz (Class 4 lasers)
  • Spectral Detection Range of 400nm – 720nm
  • Detectors: 3 HyD (hybrid detectors) Gated, 2 PMTs, and a TLD (transmitted light detector)
  • Excitation Modulation (Filters): up to 8 channels (filters multi-photon ext. & ems.); Acousto Optical Beam Splitter (AOBS): provides selectivity between 0.6 & 2 nm wavelength (no filter set needed)
  • Resonant Scanner 8kHz
  • Accelerated Z-stacking with the SuperZ galvanometer substage
  • 3-D STED (up to 70 nm in Z)
  • HyVolution – SuperResolution for every experiment and every sample (down to 140nm in XY)
Filter set for eyepiece only
Triplet (DAPI.;GFP.;DeS.R.)


Lens (Objective)

HC PL FLUOTAR 10x/0.30Plan Fluotar objective, for use with and without coverglass, no immersion. Free working distance: 11.0 mm

HC PL APO 20x/0.75 CS2Plan apochromat objective, no immersion. Free working distance: 0.62 mm

HC PL APO 63x/1.40 OIL CS2Plan apochromat oil immersion objective. Free working distance: 0.14 mm

C PL APO 93X/1.30 GLYC motCORR  STED objective with glycerol immersionMotorized correction collarFree working distance: 0.3 mm

HC PL APO 100x/1.40 Oil STED onlyPlan apochromat oil immersion objective especially designed for STED. Free working distance: 0.13 mm


Technical details and protocols