Technical details and protocols FACSAria
Excitation optics
4 lasers (488 nm, 405 nm, 561nm and 633 nm), 10 colors (5+3+2 configuration)
Forward Scatter: 488/10
Blue Laser: 695/40, 530/30
Dyes: FITC, Alexa Fluor® 488, Brilliant™ Blue 515 (BB515), DRAQ5™, 7-AAD, Propidium Iodide (PI)
Red Laser: 660/20, 730/45, 780/60
Dyes: APC or Alexa Fluor 647, APC-Alexa Fluor 700, APC-Cy7, APC-Alexa Fluor 750
Violet Laser: 450/50, 525/50, 610/20, 660/20, 710/50, 780/60
Dyes: Pacific Blue, Pacific Orange, Krome Orange
Yellow/Green Laser: 610/20, 670/14, 710/50, 780/60
Dyes: Cy3™, PE, Rhodamine
Nozzles 70, 85, 100, removable and can be sonicated.
Instructions
Quick reference guide: https://www.beckmancoulter.com/wsrportal/bibliography?docname=Gallios%20Quick%20Reference%20%28A75199AA%29.pdf
http://www.bdbiosciences.com/us/instruments/research/cell-sorters/bd-facsaria-iii/m/744763/overview
Manual
