ViiA7
Location: Main Tzabam, building C, room 004
Description
A Real-Time PCR that provides high sensitivity, high specificity, and a broad dynamic range.
96-well standard (10–100 μL reactions)
96-well Fast (10–30 μL reactions)
384-well (5–20 μL reactions)
TaqMan® Array Micro Fluidic Cards (~1 μL reactions)
Features/Specifications
- Fully Compatible with TaqMan® Array Micro Fluidic Cards, Applied Biosystems® TaqMan® Assays are the gold standard in qPCR analysis, providing high sensitivity, high specificity, and a broad dynamic range.
- TaqMan® Array-ready: Hundreds of customizable and predefined TaqMan®Array Micro Fluidic Cards are available, containing preloaded TaqMan® Gene Expression or MicroRNA Assays in an easy-to-use format. You can analyze 1-8 samples in parallel using your choice of 12-384 TaqMan® Assays.
- 384-well throughput without robotics: With TaqMan® Array Micro Fluidic Cards, there’s no need for liquid-handling robotics or complex pipetting to load; simply add your sample and master mix and run on the ViiA™ 7 System.
- Compatible with the full range of TaqMan® Assays: The ViiA™ 7 System is optimized to enable clear, clean data for all TaqMan® Assays, including MicroRNA Assays, Protein Assays, Long Non-coding RNA Assays, and Pri-miRNA Assays.
- The ViiA™ 7 System features the new OptiFlex™ System, which provides enhanced fluorescence detection enabling accurate and sensitive data analysis.
- Better accuracy: High-resolution detection system for more accurate reads with TaqMan® Array Micro Fluidic Cards, 384-well plates, and 96-well plates.
- Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities.
- Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage.
- Precise quantification: Detect as small as 1.5-fold changes in target quantities in singleplex reactions.
| Temperature Uniformity | ±0.5°C, within 30 sec of reaching temperature set point |
| Run Time | ~35 min for 96/384-well blocks, using Applied Biosystems® Fast Master Mix |
| Supported Blocks and Volume | 96-well standard (10–100 μL reactions) 96-well Fast (10–30 μL reactions) 384-well (5–20 μL reactions) TaqMan® Array Micro Fluidic Cards (~1 μL reactions) |
| Demonstrated Sensitivity | Down to 1 copy |
| Excitation/Detection Wavelengths | 6 excitation (450–670 nm) 6 emission (500–720 nm) |
| Dynamic Range | Up to 9 logs of linear dynamic range |
| Resolution | Detect as little as 1.5-fold changes in target quantities in singleplex reaction |
Tutoring
First time users must order a tutoring session.
Site
