Laser Microdissection 7
Location: Building E, room 006C (Dark room)
Contact person: Dr. Michael Assa
Booking
Description
Laser-microdissection (LMD) is a method to isolate cells, single cells, even sub-cellular moieties, under direct microscopic visualization. Thus, LCM can enrich and purify specific targets of interest for downstream applications such as DNA genotyping, RNA-SEQ, cDNA library generation, proteomics, or for cell cloning.
Manufacturer Features
- Manufacturer: Leica
- Model: LMD7 - Upright microscope
- Cutting-Laser 349 nm , Pulse frequency 10-5000 Hz, Pulse length < 4 ns and Max. pulse energy 120 µJ
- Camera Leica DFC7000 T
- Fully motorized
- HXP lamp illuminations
| Lens (Objective) | Filters sets |
|---|---|
| HCX "PL FLUOTAR" 1.25x/0.04 | Brightfield and Phase Contrast |
| UVI "MICRODISSECTION" 5x/0.12 WD 11.7mm | LMD-BGR + illumination filter wheel (triplets dye filter) |
| UVI "MICRODISSECTION" 10x/0.25 WD 11.7mm | GFP (EGfp and near range dye spectrum) |
| HCX "PL FLUOTAR" 20x/0.4 PH1 WD 6.0 mm | RHOD (Rhodamine red and near range dye spectrum) |
| HCX "PL FLUOTAR" 40x/0.6 PH1 | Y5 (CY5 and near range dye spectrum) |
| HCX "PL FLUOTAR" 63x/0.7 PH1 | |
| HCX "PL FLUOTAR MD" 150x/0.9 Dry contact |
Technical details and protocols
