Step-One Plus
Location: Main Tzabam, building C, room 004
Description
Real-Time PCR System that offers Fast runs (<40 min) and standard runs (<2 hr). Innovative VeriFlex™ Block for convenient PCR optimization and includes software capable of collecting melt curve data for High Resolution Melt (HRM) applications.
Features/Specifications
| VeriFlex™ Block | 25°C (5°C zone-to-zone) |
| Thermal Cycling System | Peltier-based system |
| Block Format | 96-well block |
| Compatible Consumables | • 96-well (0.1 mL) plates with optical flat caps • 96-well (0.1 mL) plates with optical adhesive cover • 8-tube (0.1 mL) strips with optical flat caps • Individual (0.1 mL) tubes with optical flat caps |
| Supported Volumes | 10–30 µl Note: For standard curve experiments, 40 µl in standard mode is validated. |
| Sample Ramp Rate | • Fast mode: +/- 2.2 °C/sec • Standard mode: +/- 1.6°C/sec |
| Peak Block Ramp Rate | 4.6 °C/sec |
| Temperature Range | 4°C-100°C |
| Temperature Accuracy | +/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start. |
| Temperature Uniformity | +/-0.50°C, 30 seconds after clock start. |
| Melt Curve Resolution | As small as 0.1°C |
| Optical System | Blue LED excitation source, four-emission filters, and photodiode |
| Calibrated Dyes at Installation | FAM™, SYBR® Green I, VIC®, JOE™, NED, TAMRA, ROX™ dyes |
| Passive Reference Dyes | ROX™ or any calibrated dye. Option exists to select no passive reference. |
| Data Collection | Data collected in all 3 filters for all wells regardless of plate setup. |
| Quantitative PCR Run Time | Standard cycling: Less than 2 hrs Fast cycling: Less than 40 minutes |
- The StepOnePlus™ System supports many Real-Time PCR applications, including the following:
- SNP Genotyping
- Gene Expression Analysis
- MicroRNA Expression
- Protein Expression
- Translocation Analysis
- Gene Detection
- Viral Load Analysis
The included StepOne™ Software supports a variety of analysis methods, including the following:
- Standard Curve (absolute quantitation)
- Relative Standard Curve
- Comparative Ct (ΔΔCt) (relative quantitation)
- Genotyping and Presence⁄Absence
- Melt Curve Analysis (as a standalone application)
- High Resolution Melting (as a standalone application)
Tutoring: First time users must order a tutoring session.
